Product Info Summary
SKU: | A00824 |
---|---|
Size: | 100ul |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | IF, ICC, WB |
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Product info
Product Name
Anti-Retinoid isomerohydrolase RPE65 Antibody
SKU/Catalog Number
A00824
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Retinoid isomerohydrolase RPE65 Antibody catalog # A00824. Tested in WB,ICC/IF applications. This antibody reacts with Human,Mouse,Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Retinoid isomerohydrolase RPE65 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00824)
Host
Rabbit
Contents
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from human PKC delta around the phosphorylation site of Y64.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A00824 is reactive to RPE65 in Human, Mouse, Rat
Applications
A00824 is guaranteed for IF, ICC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
60.948kDa
Background of RPE65
Collagen type III occurs in most soft connective tissues along with type I collagen. Involved in regulation of cortical development. Is the major ligand of GPR56 in the developing brain and binding to GPR56 inhibits neuronal migration and activates the RhoA pathway by coupling GPR56 to GNA13 and possibly GNA12.
Arja Lamberg, J. Biol. Chem., May 1996; 271: 11988.
GW Conrad, J. Cell Biol., Mar 1980; 84: 501.
DR Keene, J. Cell Biol., Nov 1987; 105: 2393.
HM Wang, J. Histochem. Cytochem., Nov 1980; 28: 1215.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:500-1:2000
ICC/IF: 1:50-1:200
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blotting validation for Anti-Retinoid isomerohydrolase RPE65 Antibody A00824
Western blot analysis of extracts of various cellsProtein Target Info & Infographic
Gene/Protein Information For RPE65 (Source: Uniprot.org, NCBI)
Gene Name
RPE65
Full Name
Retinoid isomerohydrolase
Weight
60.948kDa
Superfamily
carotenoid oxygenase family
Alternative Names
All-trans-retinyl-palmitate hydrolase; EC 3.1.1.64; EC 5.2.1.7; LCA2p63; mRPE65; RBP-binding membrane protein; rd12; Retinal pigment epithelium-specific 65 kDa protein; retinal pigment epithelium-specific protein (65kD); retinal pigment epithelium-specific protein 65kDa; retinitis pigmentosa 20 (autosomal recessive); retinoid isomerohydrolase; Retinol isomerase; RP20; sRPE65 RPE65 BCO3, LCA2, RP20, mRPE65, p63, rd12, sRPE65 retinoid isomerohydrolase RPE65 retinoid isomerohydrolase|BCO family, member 3|RBP-binding membrane protein|RPE65, retinoid isomerohydrolase|all-trans-retinyl-palmitate hydrolase|lutein isomerase|meso-zeaxanthin isomerase|retinal pigment epithelium specific protein 65|retinal pigment epithelium-specific 65 kDa protein|retinal pigment epithelium-specific protein 65kDa|retinitis pigmentosa 20 (autosomal recessive)|retinol isomerase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on RPE65, check out the RPE65 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for RPE65: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Retinoid isomerohydrolase RPE65 Antibody (A00824)
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No publications found for A00824
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